isolation, identification and antimicrobial sensitivity of ornithobacterium rhinotracheale in broilers chicken flocks of khuzestan, iran
نویسندگان
چکیده
ornithobacterium rhinotracheale (ort) is a bacterium associated with respiratory disease, growth retardation, decreased egg production and mortality in chickens and turkeys. the objective of this study was isolation, identification and evaluation of antimicrobial susceptibility of ort bacterium in slaughtered broilers chicken flocks based on cultural and molecular tests in khuzestan province, south-west of iran. a total of 210 tracheal swab samples were collected from 21 broiler flocks slaughtered in abattoirs of the province. the results of cultural and biochemical tests showed that 23 (10.95%) isolates from tracheal swabs of 4 flocks (19.04%) were identified as ort, but according to molecular characterization, 18 (8.57%) ort isolates were positive in pcr assay and produced the predicted 784 bp amplification product. finally, using the disk diffusion method, the drug resistance patterns of ort isolates were determined against a panel of commonly used antimicrobial agents. antimicrobial susceptibility test revealed that all isolates (100%) were sensitive to tetracycline, florfenicol and cephalexin. the highest antimicrobial resistance (89.00%) was seen for fosfomycin, sultrim and gentamicin. the results of present research showed that there was significant difference between the isolation rates of ort from various areas of the province. as well, our findings indicated that the simultaneous use of both cultural and molecular techniques results in more comprehensive outcomes in the isolation and identification of the organismfrom understudy hosts.
منابع مشابه
Isolation, identification and antimicrobial sensitivity of Ornithobacterium rhinotracheale in broilers chicken flocks of Khuzestan, Iran
Ornithobacterium rhinotracheale (ORT) is a bacterium associated with respiratory disease, growth retardation, decreased egg production and mortality in chickens and turkeys. The objective of this study was isolation, identification and evaluation of antimicrobial susceptibility of ORT bacterium in slaughtered broilers chicken flocks based on cultural and molecular tests in Khuzestan pr...
متن کاملIsolation, identification and antimicrobial sensitivity of Ornithobacterium rhinotracheale in broilers chicken flocks of Khuzestan, Iran
Ornithobacterium rhinotracheale (ORT) is a bacterium associated with respiratory disease, growth retardation, decreased egg production and mortality in chickens and turkeys. The objective of this study was isolation, identification and evaluation of antimicrobial susceptibility of ORT bacterium in slaughtered broilers chicken flocks based on cultural and molecular tests in Khuzestan province, s...
متن کاملIsolation, identification and antibiotic sensitivity determination of Ornithobacterium rhinotracheale in slaughtering broiler chicken flocks of Guilan province
Ornithobacterium rhinotracheale (ORT), a species of bacteria, is known to be associated with respiratorydisease, growth retardation, mortality and decreased egg production. The aim of the present study was todetermine seroprevalence of the infection and to identify the microorganism by bacteriological and molecularmethods. In this study, 460 serum samples and also 290 tracheal swabs were collec...
متن کاملisolation, identification and antibiotic sensitivity determination of ornithobacterium rhinotracheale in slaughtering broiler chicken flocks of guilan province
ornithobacterium rhinotracheale (ort), a species of bacteria, is known to be associated with respiratorydisease, growth retardation, mortality and decreased egg production. the aim of the present study was todetermine seroprevalence of the infection and to identify the microorganism by bacteriological and molecularmethods. in this study, 460 serum samples and also 290 tracheal swabs were collec...
متن کاملReport on isolation and identification of Ornithobacterium rhinotracheale from broiler flocks in İzmir, Turkey Bericht über Isolierung und Identifizierung von Ornithobacterium rhinotracheale aus Masthähnchenherden in İzmir, Türkei
Respiratory disease conditions continue to cause heavy economic losses in the poultry industry. They are usually caused by viral, bacterial, mycotic or parasitic agents and the course of the diseases can be influenced by environmental conditions and management failure (JOUBERT et al., 1999). At the beginning of 90`s bacteriological examinations from turkeys and chickens suffering from respirato...
متن کاملIsolation, Identification and Antimicrobial Resistance Patterns of E. coli Isolated from Chicken Flocks
Fifty E. coli strains isolated from chicken flocks were analyzed to determine their resistance to antimicrobial agents used in Tehran poultry industry. By using Mast Diagnostic kit only O6 serotype was identified. Multiple resistance to antibiotics was observed in all isolates. The highest rate of resistance was against Tetracycline (94%), followed by Rifampicin (90%), and Oxytetracycline (80%)...
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عنوان ژورنال:
veterinary research forumجلد ۷، شماره ۴، صفحات ۳۳۷-۳۴۲
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